After researching many, many stories/information about what to add into an art portfolio, I've come across everyone who says "Stay away from anime-manga style."
After much contemplating and so much thought, you should not put any anime-manga style art work in your portfolio UNLESS it has something to do with your field of study. BUT, it should not have any fan art. NO FAN ART.
Many wonder why anime-manga style is looked down on so much, and even I wonder that. So what exactly is art? How can it be measured?
Is it how "beautiful" it is? How colorful it is? How the composition fits? How the value brings depth? Or perhaps, maybe even the meaning behind the image? Anime-manga should not be an off limit style, BUT it should not be your only style.
I'm only generalizing now. Most anime-manga style artists draw only anime-manga style. They don't branch out, but that's because we're comfortable here. I know it's not just us, but I have to admit, I'm like this too. Until I was forced to draw still life and observe from life, I really only drew anime-manga style. If you want to improve, drawing from life WILL help you. It will take a long time for you to improve, but you will.
If you're fine the way you are, continue doing what you've always been doing.
Many teachers and professors don't like anime-manga (or cartoon in general) style simply because they think it's "easy" and because you don't have anything to compare it to. It can't be measured since it's your imagination. When it's still life, portrait, or landscape (from life), it can be compared. Did you get the shapes right? How about the values? Oh, you set up the composition perfectly. You see? That's why teachers and professors alike don't like things from your imagination because they cannot grade it (correctly anyway).
Chin up young fellow artists.
What is art to you?
Answer that question and go with it.